FOXP3 anticorps

N° du produit ABIN6940263

Détail du produit anti-FOXP3 anticorps

Antigène: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivité: Humain, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FOXP3 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Specificité: Recognizes a protein of 47-55 kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length FOXP3 protein

Clone: FXP3-197

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Tonsil or Testis.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur FOXP3

Antigène: FOXP3 (Forkhead Box P3 (FOXP3))

Autre désignation: FOXP3 (FOXP3 Produits)

Synonymes: anticorps foxp3, anticorps FOXP3, anticorps AIID, anticorps DIETER, anticorps IPEX, anticorps PIDX, anticorps XPID, anticorps JM2, anticorps scurfin, anticorps sf, anticorps RGD1562112, anticorps forkhead box P3, anticorps forkhead box P3a, anticorps forkhead box P3 L homeolog, anticorps FOXP3, anticorps foxp3a, anticorps foxp3, anticorps foxp3.L, anticorps Foxp3

Poids moléculaire: 47-55kDa

ID gène: 50943

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation