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POLR2A/RPB1 anticorps (pSer5)

POLR2A Reactivité: Humain, Souris, Rat WB, IHC, IF, FACS, StM Hôte: Souris Monoclonal CTD4H8 unconjugated
N° du produit ABIN6940331
  • Antigène Voir toutes POLR2A/RPB1 (POLR2A) Anticorps
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    Épitope
    • 16
    • 16
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer5
    Reactivité
    • 88
    • 46
    • 38
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 72
    • 16
    • 1
    • 1
    • 1
    Souris
    Clonalité
    • 56
    • 35
    Monoclonal
    Conjugué
    • 53
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    Cet anticorp POLR2A/RPB1 est non-conjugé
    Application
    • 48
    • 25
    • 24
    • 20
    • 12
    • 10
    • 8
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogène
    Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
    Clone
    CTD4H8
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product POLR2A Anticorps primaire
  • Indications d'application

    Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    Autre désignation
    POLR2A (POLR2A Produits)
    Synonymes
    anticorps NCU02103.1, anticorps 19.m02399, anticorps POLR2A, anticorps 220kDa, anticorps Rpb1, anticorps Rpo2-1, anticorps POLR2, anticorps POLRA, anticorps RPB1, anticorps RPBh1, anticorps RPO2, anticorps RPOL2, anticorps RpIILS, anticorps hRPB220, anticorps hsRPB1, anticorps DNA-directed RNA polymerase II largest subunit, anticorps DNA-directed RNA polymerase II subunit RPB1, anticorps RNA polymerase II large subunit Rpb1, anticorps RNA polymerase II subunit A, anticorps DNA-directed RNA polymerase II largest subunit, putative, anticorps polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, anticorps polymerase (RNA) II (DNA directed) polypeptide A, anticorps NCU02103, anticorps BBOV_I003360, anticorps EDI_169920, anticorps CMU_000800, anticorps SJAG_01713, anticorps MGYG_01527, anticorps POLR2A, anticorps TP03_0185, anticorps EBI_22514, anticorps CGB_E5020W, anticorps polr2a, anticorps Polr2a
    Sujet
    RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
    Poids moléculaire
    192-253kDa
    ID gène
    5430
    UniProt
    P24928
    Pathways
    Regulatory RNA Pathways
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