POU2AF1 anticorps (AA 148-255)
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- Antigène Voir toutes POU2AF1 Anticorps
- POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
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Épitope
- AA 148-255
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp POU2AF1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
- Clone
- BOB1-2424
- Isotype
- IgG2b kappa
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- Indications d'application
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Positive Control: Raji, Ramos cells. Spleen or Tonsil.
Known Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
- Autre désignation
- POU2AF1 (POU2AF1 Produits)
- Synonymes
- anticorps BOB1, anticorps OBF-1, anticorps OBF1, anticorps OCAB, anticorps BOB-1, anticorps BOB.1, anticorps Bob-1, anticorps Bob1, anticorps OBF.1, anticorps OCA-B, anticorps POU class 2 associating factor 1, anticorps POU domain, class 2, associating factor 1, anticorps POU2AF1, anticorps Pou2af1
- Sujet
- BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
- Poids moléculaire
- 35kDa
- ID gène
- 5450
- UniProt
- Q16633
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