EGLN1 anticorps (AA 1-24)

N° du produit ABIN6940360

Détail du produit anti-EGLN1 anticorps

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Épitope: AA 1-24

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGLN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Residues 1-24 of PHD2

Clone: 366G-76-3

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF7 cells. Brain or adrenal gland.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur EGLN1

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Autre désignation: EGLN1 (EGLN1 Produits)

Sujet: 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

Poids moléculaire: 46kDa

ID gène: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, L'effet Warburg