EGLN1 anticorps (AA 1-24)
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- Antigène Voir toutes EGLN1 Anticorps
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
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Épitope
- AA 1-24
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp EGLN1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Residues 1-24 of PHD2
- Clone
- 366G-76-3
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product EGLN1 Anticorps primaire
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- Indications d'application
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Positive Control: MCF7 cells. Brain or adrenal gland.
Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))
- Autre désignation
- EGLN1 (EGLN1 Produits)
- Sujet
- 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
- Poids moléculaire
- 46kDa
- ID gène
- 54583
- UniProt
- Q9GZT9
- Pathways
- cAMP Metabolic Process, L'effet Warburg
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