Perforin 1 anticorps (AA 413-552)

N° du produit ABIN6940388

Détail du produit anti-Perforin 1 anticorps

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Épitope: AA 413-552

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Perforin 1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)

Clone: PRF1-2470

Isotype: IgG2c kappa

Information d'application

Indications d'application:

Positive Control: HDLM-2 cells. Human spleen tissue (IHC).

Known Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Perforin 1

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Autre désignation: PRF1 (PRF1 Produits)

Synonymes: anticorps FLH2, anticorps HPLH2, anticorps P1, anticorps PFN1, anticorps PFP, anticorps PRF1, anticorps Pfn, anticorps Pfp, anticorps Prf-1, anticorps Cyta, anticorps RATCYTA, anticorps LOC443187, anticorps perforin, anticorps prf1, anticorps cytolysin, anticorps perforin-1, anticorps perforin-1-like, anticorps perforin 1, anticorps perforin 1 (pore forming protein), anticorps perforin, anticorps perforin 1 L homeolog, anticorps PRF1, anticorps Prf1, anticorps LOC443187, anticorps prf1, anticorps prf1.L

Sujet: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

Poids moléculaire: 75kDa

ID gène: 5551

UniProt: P14222

Pathways: Apoptose, Caspase Cascade in Apoptosis