RAD51 anticorps (AA 1-134)

N° du produit ABIN6940476

Détail du produit anti-RAD51 anticorps

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Épitope: AA 1-134

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD51 est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)

Clone: RAD51-2702

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: K562, HeLa, MCF-7 cells. Colon or breast Carcinoma.

Known Application: Flow Cytometry (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur RAD51

Antigène: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Autre désignation: RAD51 (RAD51 Produits)

Synonymes: anticorps BRCC5, anticorps HRAD51, anticorps HsRad51, anticorps HsT16930, anticorps MRMV2, anticorps RAD51A, anticorps RECA, anticorps CRAD51, anticorps wu:fb38e12, anticorps zgc:77754, anticorps RGD1563603, anticorps ATRAD51, anticorps F22D1.20, anticorps F22D1_20, anticorps RAS associated with diabetes protein 51, anticorps AV304093, anticorps Rad51a, anticorps Reca, anticorps brcc5, anticorps rad51, anticorps rad51a, anticorps reca, anticorps xrad51, anticorps RAD51 recombinase, anticorps RAS associated with diabetes protein 51, anticorps RAD51 recombinase L homeolog, anticorps RAD51, anticorps rad51, anticorps Rad51, anticorps rad51.L

Sujet: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies.RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.

Poids moléculaire: 37kDa

ID gène: 5888

UniProt: Q06609

Pathways: Réparation de l'ADN