Cyclin D1 anticorps
-
- Antigène Voir toutes Cyclin D1 (CCND1) Anticorps
- Cyclin D1 (CCND1)
-
Reactivité
- Humain, Rat, Souris, Singe
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp Cyclin D1 est non-conjugé
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
- Specificité
- Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40 % of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
- Purification
- Purified by Protein A/G
- Immunogène
- Human full length recombinant cyclin D1 protein
- Clone
- DCS-6
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CCND1 Anticorps primaire
-
-
- Indications d'application
-
Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
-
- Antigène
- Cyclin D1 (CCND1)
- Autre désignation
- CCND1 (CCND1 Produits)
- Synonymes
- anticorps BCL1, anticorps D11S287E, anticorps PRAD1, anticorps U21B31, anticorps AI327039, anticorps CycD1, anticorps Cyl-1, anticorps bcl-1, anticorps cD1, anticorps cb161, anticorps cycd1, anticorps etID37810.7, anticorps fb52e01, anticorps fc45c08, anticorps fc83a12, anticorps id:ibd1198, anticorps wu:fb52e01, anticorps wu:fc45c08, anticorps wu:fc83a12, anticorps ccnd1b, anticorps prad1, anticorps ccnd1, anticorps ccnd1a, anticorps bcl1, anticorps d11s287e, anticorps u21b31, anticorps CCND1, anticorps cyclin D1, anticorps cyclin D1 S homeolog, anticorps cyclin D1 L homeolog, anticorps CCND1, anticorps Ccnd1, anticorps ccnd1, anticorps ccnd1.S, anticorps ccnd1.L
- Poids moléculaire
- 36kDa
- ID gène
- 595
- UniProt
- P24385
- Pathways
- Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
-