VISTA anticorps
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- Antigène Voir toutes VISTA Anticorps
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp VISTA est non-conjugé
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Application
- ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant full-length human VISTA protein
- Clone
- VISTA-3007
- Isotype
- IgG2b kappa
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- Indications d'application
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Positive Control: Spleen or Placenta.
Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
- Autre désignation
- VSIR (VISTA Produits)
- Synonymes
- anticorps MGC112715, anticorps MGC151567, anticorps B7-H5, anticorps B7H5, anticorps GI24, anticorps PP2135, anticorps SISP1, anticorps Dies1, anticorps VISTA, anticorps V-set immunoregulatory receptor, anticorps vsir, anticorps Vsir, anticorps VSIR
- Sujet
- VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
- Poids moléculaire
- 34kDa
- ID gène
- 64115
- UniProt
- Q9H7M9
- Pathways
- Cancer Immune Checkpoints
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