Sialoadhesin/CD169 anticorps (N-Term)

N° du produit ABIN6940605

Détail du produit anti-Sialoadhesin/CD169 anticorps

Antigène: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Épitope: N-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Sialoadhesin/CD169 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.

Clone: HSn 7D2

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Human spleen, human peripheral blood monocytes or MCF7 cell.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Sialoadhesin/CD169

Antigène: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Autre désignation: SIGLEC1 (SIGLEC1 Produits)

Synonymes: anticorps Cd169, anticorps Siglec-1, anticorps Sn, anticorps CD169, anticorps SIGLEC-1, anticorps SN, anticorps dJ1009E24.1, anticorps SA, anticorps SIGLEC1, anticorps p210, anticorps pSn, anticorps sialic acid binding Ig like lectin 1, anticorps sialic acid binding Ig-like lectin 1, sialoadhesin, anticorps SIGLEC1, anticorps Siglec1

Sujet: Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1,709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells.

Poids moléculaire: 185kDa

ID gène: 6614