SPI1 anticorps (AA 16-170)
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- Antigène Voir toutes SPI1 Anticorps
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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Épitope
- AA 16-170
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp SPI1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
- Clone
- PU1-2146
- Isotype
- IgG2b kappa
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- Indications d'application
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Positive Control: Ramos, K-562 cells, THP-1 cells. Lymph Node, Spleen, Hodgkin's Lymphoma, Colon Carcinoma.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- Autre désignation
- SPI1 (SPI1 Produits)
- Synonymes
- anticorps OF, anticorps PU.1, anticorps SFPI1, anticorps SPI-1, anticorps SPI-A, anticorps Dis-1, anticorps Dis1, anticorps Sfpi-1, anticorps Sfpi1, anticorps Spi-1, anticorps Tcfpu1, anticorps Tfpu.1, anticorps Pu.1, anticorps cb1086, anticorps pu.1, anticorps pu1, anticorps sfpi1, anticorps si:by184l24.2, anticorps spi1, anticorps SPI1/PU.1, anticorps spi1l, anticorps zgc:152991, anticorps Spi-1 proto-oncogene, anticorps spleen focus forming virus (SFFV) proviral integration oncogene, anticorps Spi-1 proto-oncogene b, anticorps Spi-1 proto-oncogene a, anticorps SPI1, anticorps Spi1, anticorps spi1b, anticorps spi1a
- Sujet
- PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
- Poids moléculaire
- 40kDa
- ID gène
- 6688
- UniProt
- P17947
- Pathways
- Stem Cell Maintenance
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