BRCA1 anticorps (AA 445-620)

N° du produit ABIN6940661

Détail du produit anti-BRCA1 anticorps

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Épitope: AA 445-620

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRCA1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant human BRCA1 protein fragment (around aa445-620) (exact sequence is proprietary)

Clone: BRCA1-1398

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: A431 or HeLa cells. Breast, Ovarian or Renal Cell Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (2-5 μg/mL for 60 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur BRCA1

Antigène: BRCA1 (Breast Cancer 1 (BRCA1))

Autre désignation: BRCA1 (BRCA1 Produits)

Sujet: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Poids moléculaire: 220kDa

ID gène: 672

UniProt: P38398

Pathways: Cycle Cellulaire, Réparation de l'ADN, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus