TYMS anticorps (AA 60-174)
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- Antigène Voir toutes TYMS Anticorps
- TYMS (Thymidylate Synthetase (TYMS))
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Épitope
- AA 60-174
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp TYMS est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)
- Specificité
- It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
- Clone
- TYMS-1884
- Isotype
- IgG2c kappa
- Top Product
- Discover our top product TYMS Anticorps primaire
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- Indications d'application
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Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- TYMS (Thymidylate Synthetase (TYMS))
- Autre désignation
- TYMS (TYMS Produits)
- Classe de substances
- Viral Protein
- Poids moléculaire
- 36kDa
- ID gène
- 7298
- UniProt
- P04818
- Pathways
- Mitotic G1-G1/S Phases
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