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TYMS anticorps (AA 60-174)

TYMS Reactivité: Humain WB, IF, FACS, IHC, ISt, RIA, StM Hôte: Souris Monoclonal TYMS-1884 unconjugated
N° du produit ABIN6940822
  • Antigène Voir toutes TYMS Anticorps
    TYMS (Thymidylate Synthetase (TYMS))
    Épitope
    • 15
    • 12
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 60-174
    Reactivité
    • 92
    • 29
    • 14
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 50
    • 41
    • 1
    Souris
    Clonalité
    • 48
    • 44
    Monoclonal
    Conjugué
    • 46
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TYMS est non-conjugé
    Application
    • 51
    • 41
    • 33
    • 23
    • 19
    • 18
    • 17
    • 17
    • 14
    • 13
    • 9
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)
    Specificité
    It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
    Clone
    TYMS-1884
    Isotype
    IgG2c kappa
    Top Product
    Discover our top product TYMS Anticorps primaire
  • Indications d'application

    Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    TYMS (Thymidylate Synthetase (TYMS))
    Autre désignation
    TYMS (TYMS Produits)
    Classe de substances
    Viral Protein
    Poids moléculaire
    36kDa
    ID gène
    7298
    UniProt
    P04818
    Pathways
    Mitotic G1-G1/S Phases
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