TYR anticorps

N° du produit ABIN6940824

Détail du produit anti-TYR anticorps

Antigène: TYR (Tyrosinase (TYR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TYR est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Specificité: Recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

Purification: Purified by Protein A/G

Immunogène: Recombinant tyrosinase protein

Clone: T311

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells. Melanoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur TYR

Antigène: TYR (Tyrosinase (TYR))

Autre désignation: TYR (TYR Produits)

Synonymes: anticorps sandy, anticorps zgc:109705, anticorps TYR, anticorps TYRO, anticorps tyr, anticorps LOC100136546, anticorps CMM8, anticorps OCA1A, anticorps OCAIA, anticorps SHEP3, anticorps albino, anticorps c, anticorps skc35, anticorps C, anticorps tyrosinase, anticorps TYRosinase, anticorps tyrosinase S homeolog, anticorps tyr, anticorps tyr-5, anticorps tyr.S, anticorps TYR, anticorps Celal_3121, anticorps Halhy_4121, anticorps Mesop_2736, anticorps LOC100136546, anticorps Tyr

Poids moléculaire: 70-80kDa

ID gène: 7299

UniProt: P14679