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Vimentin anticorps

VIM Reactivité: Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre WB, IHC, FACS, StM Hôte: Souris Monoclonal VM452 unconjugated
N° du produit ABIN6940879
  • Antigène Voir toutes Vimentin (VIM) Anticorps
    Vimentin (VIM)
    Reactivité
    • 305
    • 121
    • 118
    • 60
    • 59
    • 50
    • 42
    • 37
    • 30
    • 21
    • 19
    • 14
    • 13
    • 8
    • 8
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre
    Hôte
    • 158
    • 130
    • 11
    • 6
    • 6
    • 5
    • 2
    Souris
    Clonalité
    • 172
    • 145
    Monoclonal
    Conjugué
    • 198
    • 15
    • 13
    • 12
    • 9
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp Vimentin est non-conjugé
    Application
    • 263
    • 114
    • 104
    • 98
    • 88
    • 64
    • 48
    • 40
    • 39
    • 33
    • 23
    • 20
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
    Specificité
    This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
    Aucune reactivité croisée
    Souris, Rat (Rattus)
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant full-length human vimentin protein
    Clone
    VM452
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product VIM Anticorps primaire
  • Indications d'application

    Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

    Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Vimentin (VIM)
    Autre désignation
    VIM (VIM Produits)
    Poids moléculaire
    57-60kDa
    ID gène
    7431
    UniProt
    P08670
    Pathways
    Caspase Cascade in Apoptosis
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