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Vimentin anticorps

Name: Vimentin anticorps
Brand: antibodies-online
SKU: ABIN6940879
Price: 663.89 EUR
Availability: InStock

VIM Reactivité: Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre WB, IHC, FACS, StM Hôte: Souris Monoclonal VM452 unconjugated

Western Blot Analysis Raji cell lysate Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin anticorps)

Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (Vimentin anticorps)

PAr

Flow Cytometry of human Vimentin on Jurkat cells. (Vimentin anticorps)

FACS

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin anticorps)

IHC

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin anticorps)

SDS

Western Blot Analysis of human A375 cell lysate using Vimentin Mouse Monoclonal Antibody (VM452). (Vimentin anticorps)

6 images

N° du produit ABIN6940879

Fiche de données

Antigène Voir toutes Vimentin (VIM) Anticorps

Vimentin (VIM)
Reactivité
305

121

118

60

59

50

42

37

30

21

19

14

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre
Hôte
158

130

11

6

6

5

2
Souris
Anticorps secondaires appropriés
Clonalité
172

145
Monoclonal
Conjugué
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
Cet anticorp Vimentin est non-conjugé
Application
263

114

104

98

88

64

48

40

39

33

23

20

8

5

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Specificité

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Aucune reactivité croisée

Souris, Rat (Rattus)

Purification

Purified by Protein A/G

Immunogène

Recombinant full-length human vimentin protein

Clone

VM452

Isotype

IgG1 kappa

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anti-Vimentin (VIM) antibody
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VIM Reactivité: Humain, Souris WB, IF, FACS, IHC (p) Hôte: Lapin Polyclonal RB41034 unconjugated

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VIM Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

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VIM Reactivité: Humain WB, IHC, ICC, IP Hôte: Souris Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivité: Humain WB, IF, FACS, IHC (p) Hôte: Lapin Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reactivité: Humain WB, ELISA, IF, IP Hôte: Souris Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivité: Humain WB, IHC, ELISA, FACS Hôte: Souris Monoclonal 3A1F2 unconjugated

Indications d'application

Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-80 °C

Stockage commentaire

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption

24 months
Antigène

Vimentin (VIM)

Autre désignation

VIM (VIM Produits)

Poids moléculaire

57-60kDa

ID gène

7431

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis