WT1 anticorps (AA 1-181)

N° du produit ABIN6940900

Détail du produit anti-WT1 anticorps

Antigène: WT1 (Wilms Tumor 1 (WT1))

Épitope: AA 1-181

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp WT1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificité: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Purification: Purified by Protein A/G

Immunogène: Recombinant protein corresponding to residues 1-181 of human WT1.

Clone: 6F-H2

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur WT1

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: WT1 (WT1 Produits)

Synonymes: anticorps AWT1, anticorps EWS-WT1, anticorps GUD, anticorps NPHS4, anticorps WAGR, anticorps WIT-2, anticorps WT33, anticorps CWT1, anticorps WT1, anticorps XWT1a, anticorps wt-1, anticorps wt1, anticorps xWT1, anticorps WT-1, anticorps gud, anticorps wagr, anticorps wt33, anticorps xwt1, anticorps wit-2, anticorps si:ch211-160j6.3, anticorps zgc:152697, anticorps D630046I19Rik, anticorps Wt-1, anticorps XWT1b, anticorps XeWT1, anticorps Wilms tumor 1, anticorps Wilms tumor 1 S homeolog, anticorps wilms tumor 1b, anticorps Wilms tumor protein, anticorps Wilms tumor 1 homolog, anticorps Wilms tumor 1 L homeolog, anticorps WT1, anticorps wt1.S, anticorps wt1, anticorps wt1b, anticorps Wt1, anticorps wt1.L

Poids moléculaire: 47-55kDa

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation