ZAP70 anticorps (AA 1-254)
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- Antigène Voir toutes ZAP70 Anticorps
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
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Épitope
- AA 1-254
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ZAP70 est non-conjugé
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
- Clone
- 2F3-2
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product ZAP70 Anticorps primaire
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- Indications d'application
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Positive Control: Jurkat cells. Tonsil or lymph node.
Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
- Autre désignation
- ZAP70 (ZAP70 Produits)
- Sujet
- ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive Purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
- Poids moléculaire
- 70kDa
- ID gène
- 7535
- UniProt
- P43403
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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