Calnexin anticorps (N-Term)

N° du produit ABIN6940952

Détail du produit anti-Calnexin anticorps

Antigène: Calnexin (CANX)

Épitope: AA 1-300, N-Term

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Specificité: It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Aucune reactivité croisée: Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Clone: CANX-1541

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: CANX (CANX Produits)

Poids moléculaire: 90kDa

ID gène: 821

UniProt: P27824

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis