Calnexin anticorps (N-Term)
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- Antigène Voir toutes Calnexin (CANX) Anticorps
- Calnexin (CANX)
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Épitope
- AA 1-300, N-Term
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Calnexin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)
- Specificité
- It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
- Aucune reactivité croisée
- Rat (Rattus)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
- Clone
- CANX-1541
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CANX Anticorps primaire
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- Indications d'application
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Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- Calnexin (CANX)
- Autre désignation
- CANX (CANX Produits)
- Poids moléculaire
- 90kDa
- ID gène
- 821
- UniProt
- P27824
- Pathways
- Signalisation MAPK, Thyroid Hormone Synthesis
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