Cyclin B1 anticorps

N° du produit ABIN6940989

Détail du produit anti-Cyclin B1 anticorps

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain, Souris, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Specificité: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Purification: Purified by Protein A/G

Immunogène: Hamster Cyclin B1 protein

Clone: V92-1

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein) (precipitates active CDK1/cyclin B1 complexes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: CCNB1 (CCNB1 Produits)

Synonymes: anticorps ccnb, anticorps cycb, anticorps cb267, anticorps cycb1, anticorps wu:fa19g04, anticorps wu:fb16d01, anticorps wu:fb16e07, anticorps wu:fi21c01, anticorps ccnb1, anticorps MGC53596, anticorps CCNB, anticorps Ccnb1-rs1, anticorps Ccnb1-rs13, anticorps CycB1, anticorps Cycb-4, anticorps Cycb-5, anticorps Cycb1-rs1, anticorps cyclin B1, anticorps cyclin B1 S homeolog, anticorps ccnb1, anticorps ccnb1.2, anticorps ccnb1.S, anticorps CCNB1, anticorps Ccnb1, anticorps ccnb1.2.S

Poids moléculaire: 55-62kDa

ID gène: 891

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase