CD3 epsilon anticorps

N° du produit ABIN6941023

Détail du produit anti-CD3 epsilon anticorps

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat), Immunohistochemistry (Acetone-fixed) (IHC (af))

Specificité: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Purification: Purified by Protein A/G

Immunogène: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

Clone: UCHT1

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Human peripheral blood. Jurkat cells. Tonsil or lymph node.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Acetone-fixed frozen tissues) (1-2 μg/mL for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3E (CD3E Produits)

Synonymes: anticorps cd3e, anticorps CD3E, anticorps t3e, anticorps tcre, anticorps AI504783, anticorps CD3, anticorps CD3epsilon, anticorps T3e, anticorps T3E, anticorps TCRE, anticorps CD3e molecule, anticorps CD3 antigen, epsilon polypeptide, anticorps cd3e, anticorps CD3E, anticorps Cd3e

Poids moléculaire: 20kDa

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway