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CD14 anticorps (AA 25-148)

Name: CD14 anticorps (AA 25-148)
Brand: antibodies-online
SKU: ABIN6941093
Price: 640.2 EUR
Availability: InStock

CD14 Reactivité: Humain FACS, WB, IHC, ISt, StM Hôte: Souris Monoclonal LPSR-2385 unconjugated

Flow Cytometric Analysis of A549 cells using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 anticorps (AA 25-148))

FACS

Western Blot Analysis of A549 cell lysate using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 anticorps (AA 25-148))

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD14 anticorps (AA 25-148))

PAr

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 anticorps (AA 25-148))

IHC

Formalin-fixed, paraffin-embedded human Tonsil stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 anticorps (AA 25-148))

IHC

SDS-PAGE Analysis Purified CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385). (CD14 anticorps (AA 25-148))

SDS

6 images

N° du produit ABIN6941093

Fiche de données

Antigène Voir toutes CD14 Anticorps

CD14
Épitope
8

6

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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1
AA 25-148
Reactivité
244

46

32

25

24

18

17

16

15

14

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Humain
Hôte
213

52

25

3

1
Souris
Anticorps secondaires appropriés
Clonalité
240

54
Monoclonal
Conjugué
115

31

24

18

15

8

6

5

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp CD14 est non-conjugé
Application
219

83

59

56

52

49

21

14

13

12

6

6

4

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Specificité

Recognizes a protein of 55 kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

Purification

Purified by Protein A/G

Immunogène

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Clone

LPSR-2385

Isotype

IgG1 kappa

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Indications d'application

Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-80 °C

Stockage commentaire

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption

24 months
Antigène

CD14

Autre désignation

CD14 (CD14 Produits)

Synonymes

anticorps CD14 molecule, anticorps CD14 antigen, anticorps CD14, anticorps Cd14

Poids moléculaire

55kDa

ID gène

929

UniProt

P08571

Pathways

Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades