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CD47 anticorps (AA 18-135)

Name: CD47 anticorps (AA 18-135)
Brand: antibodies-online
SKU: ABIN6941215
Price: 640.2 EUR
Availability: InStock

CD47 Reactivité: Humain FACS, IF, IHC, ELISA, StM, Coat Hôte: Souris Monoclonal CD47-3019 unconjugated

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/3019). (CD47 anticorps (AA 18-135))

IHC

SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/3019). (CD47 anticorps (AA 18-135))

SDS

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/3019) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD47 anticorps (AA 18-135))

PAr

Flow Cytometric Analysis of MCF-7 cells using CD47 Mouse Monoclonal Antibody (IAP/3019) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red). (CD47 anticorps (AA 18-135))

FACS

IHC

Immunofluorescence staining of MCF-7 cells using CD47 Mouse Monoclonal Antibody (IAP/3019) followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD47 anticorps (AA 18-135))

6 images

N° du produit ABIN6941215

Fiche de données

Antigène Voir toutes CD47 Anticorps

CD47
Épitope
16

15

8

8

7

4

4

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 18-135
Reactivité
121

52

27

6

3

2

2

2

1

1

1

1
Humain
Hôte
78

64

9

1
Souris
Anticorps secondaires appropriés
Clonalité
77

73

1
Monoclonal
Conjugué
66

13

12

7

5

5

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1
Cet anticorp CD47 est non-conjugé
Application
87

85

41

39

34

26

26

18

16

16

14

10

7

4

3

3

3

3

2

2

2

2

2

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Staining Methods (StM), Coating (Coat)

Specificité

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Purification

Purified by Protein A/G

Immunogène

A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

Clone

CD47-3019

Isotype

IgG1 kappa

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Indications d'application

Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-80 °C

Stockage commentaire

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption

24 months
Antigène

CD47

Autre désignation

CD47 (CD47 Produits)

Synonymes

anticorps IAP, anticorps MER6, anticorps OA3, anticorps 9130415E20Rik, anticorps AA407862, anticorps AI848868, anticorps AW108519, anticorps B430305P08Rik, anticorps Itgp, anticorps CD47/IAP, anticorps alkaline phosphatase, intestinal, anticorps CD47 molecule, anticorps CD47 antigen (Rh-related antigen, integrin-associated signal transducer), anticorps Cd47 molecule, anticorps ALPI, anticorps CD47, anticorps Cd47

Poids moléculaire

~50kDa

ID gène

961

UniProt

Q08722