Biotin anticorps

N° du produit ABIN6941338

Détail du produit anti-Biotin anticorps

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Biotin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)

Specificité: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Purification: Purified by Protein A/G

Immunogène: Biotinylated sheep immunoglobulin

Clone: Hyb-8

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Biotinylated proteins in solution or on tissues.

Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Biotin

Antigène: Biotin

Autre désignation: Biotin (Vitamin B7 or Vitamin H) (Biotin Produits)

Classe de substances: Chemical

Poids moléculaire: 244Da