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KRT anticorps

KRT126P Reactivité: Humain, Souris, Rat, Chien, Poulet, Boeuf (Vache), Singe, Lapin FACS, IHC, StM Hôte: Souris Monoclonal Cocktail PAN-CK unconjugated
N° du produit ABIN6941353
  • Antigène Tous les produits KRT (KRT126P)
    KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
    Reactivité
    Humain, Souris, Rat, Chien, Poulet, Boeuf (Vache), Singe, Lapin
    Hôte
    • 4
    • 3
    Souris
    Clonalité
    • 5
    • 2
    Monoclonal
    Conjugué
    • 7
    Cet anticorp KRT est non-conjugé
    Application
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificité
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
    Purification
    Purified by Protein A/G
    Immunogène
    Human epidermal keratins
    Clone
    Cocktail PAN-CK
    Isotype
    IgG1 kappa
  • Indications d'application

    Positive Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))
    Autre désignation
    KRT (KRT126P Produits)
    Synonymes
    anticorps KRT, anticorps keratin 126 pseudogene, anticorps KRT126P
    Poids moléculaire
    40-67kDa (Multiple)
    ID gène
    374454, 51350
    UniProt
    Q7Z794, Q01546
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