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Keratin 77 anticorps

KRT77 Reactivité: Humain, Rat, Souris, Lapin, Boeuf (Vache), Chien, Poulet, Singe, Tortue IHC, WB, IF, FACS, StM Hôte: Souris Monoclonal AE-1 unconjugated
N° du produit ABIN6941429
  • Antigène Voir toutes Keratin 77 (KRT77) Anticorps
    Keratin 77 (KRT77)
    Reactivité
    • 25
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris, Lapin, Boeuf (Vache), Chien, Poulet, Singe, Tortue
    Hôte
    • 18
    • 7
    Souris
    Clonalité
    • 16
    • 9
    Monoclonal
    Conjugué
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Keratin 77 est non-conjugé
    Application
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    Specificité
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
    Purification
    Purified by Protein A/G
    Immunogène
    Human epidermal keratin
    Clone
    AE-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product KRT77 Anticorps primaire
  • Indications d'application

    Positive Control: Skin or squamous cell carcinoma (IHC).

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Keratin 77 (KRT77)
    Autre désignation
    KRT77 (KRT77 Produits)
    Synonymes
    anticorps K1B, anticorps KRT1B, anticorps 4732484G22Rik, anticorps K1emb, anticorps Krt239, anticorps mK1b, anticorps Kb39, anticorps keratin 77, anticorps KRT77, anticorps Krt77
    Poids moléculaire
    40-56.5kDa
    ID gène
    374454
    UniProt
    Q7Z794
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