PMS2 anticorps
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- Antigène Voir toutes PMS2 Anticorps
- PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp PMS2 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))
- Réactivité croisée
- Humain
- Purification
- Purified by Protein A.
- Immunogène
- Full length protein
- Clone
- 9H11
- Isotype
- IgG
- Top Product
- Discover our top product PMS2 Anticorps primaire
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- Indications d'application
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WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC() - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C for 12 months.
- Date de péremption
- 12 months
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- Antigène
- PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))
- Autre désignation
- PMS2 (PMS2 Produits)
- Synonymes
- anticorps HNPCC4, anticorps PMS2CL, anticorps PMSL2, anticorps AW555130, anticorps Pmsl2, anticorps PMS1 homolog 2, mismatch repair system component, anticorps PMS1 homolog2, mismatch repair system component, anticorps mismatch repair endonuclease PMS2, anticorps PMS2, anticorps Pms2, anticorps LOC463257, anticorps LOC107984056
- Sujet
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Synonyms: Mismatch repair endonuclease PMS2, DNA mismatch repair protein PMS2, PMS1 protein homolog 2, PMS2
Background: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
- ID gène
- 5395
- UniProt
- P54278
- Pathways
- Réparation de l'ADN, Production of Molecular Mediator of Immune Response
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