PUM1 anticorps

N° du produit ABIN6944728

Détail du produit anti-PUM1 anticorps

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PUM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: Recombinant protein within human Pumilio 1 aa 1-200.

Clone: 7B2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PUM1

Antigène: PUM1 (Pumilio Homolog 1 (PUM1))

Autre désignation: PUM1 (PUM1 Produits)

Synonymes: anticorps fb96a03, anticorps pumilio1, anticorps si:ch211-285e16.1, anticorps wu:fb96a03, anticorps wu:fb96a08, anticorps zgc:63671, anticorps PUM1, anticorps DKFZp459K0627, anticorps AA517475, anticorps Pumm, anticorps mKIAA0099, anticorps HSPUM, anticorps PUMH, anticorps PUMH1, anticorps PUML1, anticorps pumilio RNA-binding family member 1, anticorps pumilio RNA binding family member 1, anticorps pum1, anticorps PUM1, anticorps Pum1

Sujet:

Synonyms: Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, PUMH, HSPUM, PUMH1, PUML1, SCA47

Background: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery. Plays a role in cytoplasmic sensing of viral infection. In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.

ID gène: 9698

UniProt: Q14671