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Histone 3 anticorps (H3K27me3)

Name: Histone 3 anticorps (H3K27me3)
SKU: ABIN6952339
Price: 601.67 EUR
Availability: InStock

ChIP-seq Grade H3 Reactivité: Humain WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, SNAP-ChIP Hôte: Lapin Polyclonal unconjugated

(1 reference)

N° du produit ABIN6952339

Fiche de données

Antigène Voir toutes Histone 3 (H3) Anticorps

Histone 3 (H3)
Épitope
61

49

47

44

41

40

36

36

35

34

34

33

32

30

28

27

26

23

23

23

22

22

20

18

18

17

16

16

16

15

15

15

15

14

13

13

13

12

12

12

11

11

10

10

10

10

9

9

8

8
H3K27me3
Reactivité
1393

958

878

59

42

41

41

40

39

35

35

31

26

22

20

6

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Humain
Hôte
1203

243

12

5
Lapin
Anticorps secondaires appropriés
Clonalité
1127

335

1
Polyclonal
Conjugué
798

80

51

50

48

48

48

48

34

33

22

18

18

18

17

17

17

17

17

17

17

17

12

1
Cet anticorp Histone 3 est non-conjugé
Application
1034

406

374

315

241

188

173

171

163

153

139

127

117

48

38

34

32

22

15

14

7

7

5

4

3

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)

Specificité

Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Réactivité croisée

Arabidopsis, Caenorhabditis elegans (C. elegans), Daphnia, Mouche des fruits (Drosophila melanogaster), Maize/Corn (Zea mays), Souris, Poplar (Populus), Red Algae (Chondrus), Tomate (Solanum lycopersicum)

Réactivité croisée (Details)

wide range

Purification

Peptide affinity purified

Classe de qualité

ChIP-seq Grade

Immunogène

synthetic peptide

Top Product

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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivité: Humain WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27ac) antibody
H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivité: Humain, Souris WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Souris Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivité: Humain, Souris WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivité: Humain, Souris, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivité: Humain WB, IHC, IF, ChIP, IP, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivité: Humain, Souris, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivité: Humain WB, IHC, ChIP, IP Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivité: Humain WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

Indications d'application

ChIP/ChIP-seq 1 - 2 μg:ChIP
ELISA 1:100 - 1:500
Dot Blot 1:5,000
WB 1:1,000
IF 1:200

Restrictions

For Research Use only
Format

Liquid

Concentration

1.1 μg/μL

Buffer

PBS, 0.05 % azide, 0.05 % ProClin300

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C
Wang, Zhao, Liu, Wang, Qiao, Yi, Jiang, Kou, Zhao, Yin, Li, Wang, Jiang, Gao, Chen: "BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4b-mediated chromosomal stability safeguarding." dans: Protein & cell, (2022) (PubMed).
Antigène

Histone 3 (H3)

Autre désignation

Histone 3 (H3 Produits)

Synonymes

anticorps H-3, anticorps histocompatibility 3, anticorps H3

Sujet

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions.

UniProt

P68431