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Histone 3 anticorps (H3K4me3)

ChIP-seq Grade H3 Reactivité: Humain WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag, PeA, SNAP-ChIP Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6952351
  • Antigène Voir toutes Histone 3 (H3) Anticorps
    Histone 3 (H3)
    Épitope
    • 61
    • 49
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    • 40
    • 36
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    • 8
    • 8
    H3K4me3
    Reactivité
    • 1394
    • 958
    • 878
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 31
    • 26
    • 22
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    • 6
    • 3
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    • 2
    • 2
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 1203
    • 244
    • 12
    • 5
    Lapin
    Clonalité
    • 1127
    • 336
    • 1
    Polyclonal
    Conjugué
    • 799
    • 80
    • 51
    • 50
    • 48
    • 48
    • 48
    • 48
    • 34
    • 33
    • 22
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    • 17
    • 12
    • 1
    Cet anticorp Histone 3 est non-conjugé
    Application
    • 1035
    • 407
    • 374
    • 315
    • 241
    • 188
    • 173
    • 171
    • 163
    • 153
    • 139
    • 127
    • 117
    • 48
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    • 32
    • 22
    • 15
    • 13
    • 7
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    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
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    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)
    Specificité
    Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide.
     Réactivité croisée
    Arabidopsis, Daphnia, Maize/Corn (Zea mays), Souris, Poplar (Populus), Riz (Oryza sativa), Tomate (Solanum lycopersicum), Truite, Poisson zèbre (Danio rerio)
    Réactivité croisée (Details)
    wide range
    Purification
    Peptide affinity purified
    Classe de qualité
    ChIP-seq Grade
    Immunogène
    synthetic peptide
    Top Product
    Discover our top product H3 Anticorps primaire
  • Indications d'application
    ChIP/ChIP-seq<1 μg:ChIP
    ELISA 1:1,000
    Dot Blot 1:10,000
    Peptde Array 1:10,000
    WB 1:1,000
    IF 1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.3 μg/μL
    Buffer
    PBS, 0.05 % azide, 0.05 % ProClin300
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Schreiber, Mercier, Jiménez, Ye, García-Sánchez, Klein, Meunier, Ghimire, Birck, Jost, de Lichtenberg, Honoré, Serup, Gradwohl: "Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network." dans: Molecular metabolism, Vol. 53, pp. 101313, (2021) (PubMed).

  • Antigène
    Histone 3 (H3)
    Autre désignation
    Histone 3 (H3 Produits)
    Synonymes
    anticorps H-3, anticorps histocompatibility 3, anticorps H3
    Sujet
    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K4 is associated with activation of gene transcription.
    UniProt
    P68431
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