Recombinant SARS-CoV-2 Nucleocapsid anticorps

N° du produit ABIN6953059

Détail du produit anti-SARS-CoV-2 Nucleocapsid anticorps

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Type d'anticorp: Recombinant Antibody

Reactivité: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV)

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp SARS-CoV-2 Nucleocapsid est non-conjugé

Application: ELISA, Immunofluorescence (IF)

Specificité: This antibody binds the amino acid residues between 11-19 of the N protein of the SARS CoV as well as SARS-CoV-2 (COVID-19) nucleocapsid protein.

Attributs du produit:

OriginalSpeciesName: Human

OriginalFormat: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by cloning the variable regions of the scFvs selected from phage display libraries into separate vectors for IgG1 heavy-chain and light-chain expression. The harvested supernatents were then purified on protein A columns. The original immunogen was the whole irradiated virion.

Clone: CR3018 (03-018)

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody is recommended for detection of SARS CoV2 protein N (nucleoprotein). This antibody binds both the nucleocapsid protein of the SARS-CoV and SARS CoV-2 (2019-nCoV). Initial characterization of the antibody for binding to 2019-nCoV was done using ELISA. This antibody shows potential to be used for development of diagnostic assays. Various isotype versions of the antibody namely human IgG1, IgG3, IgM, IgA and the less common IgG2 and IgG4 are available for the investigation of their role in response to SARS CoV2. Competitive ELISA of this antibody with CR3009 suggests that both these antibodies bind different epitopes of the N protein of SARS CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Immunofluorescence staining was used to demonstrate binding of CR3018 to SARS-CoV infected Vero cells.

Competitive ELISA of both anti-nucleocapsid antibodies suggests that they bind different, non-overlapping epitopes of the N protein of SARS-CoV. Thus, a combination of these two antibodies is suggested for virus capture assays. Clone CR3018 binds the amino acid residues between 11-19 of the N protein of SARS-CoV, while clone CR3009 binds a non-linear/conformational epitope of the N protein of SARS-CoV, both of which are sufficiently conserved to permit binding of these antibodies to SARS-CoV-2.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Références pour anticorps anti-SARS-CoV-2 Nucleocapsid (ABIN6953059)

Gaynor, Vaysburd, Harman, Albecka, Jeffrey, Beswick, Papa, Chen, Mallery, McGuinness, Van Rietschoten, Stanway, Brear, Lulla, Ciazynska, Chang, Sharp, Neary, Box, Herriott, Kijak, Tatham, Bentley et al.: "Multivalent bicyclic peptides are an effective antiviral modality that can potently inhibit SARS-CoV-2. ..." dans: Nature communications, Vol. 14, Issue 1, pp. 3583, (2023) (PubMed).

Ramm, Dondapati, Trinh, Wenzel, Walter, Zemella, Kubick: "The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins." dans: Frontiers in bioengineering and biotechnology, Vol. 10, pp. 896751, (2022) (PubMed).

van den Brink, Ter Meulen, Cox, Jongeneelen, Thijsse, Throsby, Marissen, Rood, Bakker, Gelderblom, Martina, Osterhaus, Preiser, Doerr, de Kruif, Goudsmit: "Molecular and biological characterization of human monoclonal antibodies binding to the spike and nucleocapsid proteins of severe acute respiratory syndrome coronavirus." dans: Journal of virology, Vol. 79, Issue 3, pp. 1635-44, (2005) (PubMed).

Détails sur SARS-CoV-2 Nucleocapsid

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Autre désignation: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Produits)

Synonymes: anticorps nucleocapsid phosphoprotein, anticorps N

Classe de substances: Viral Protein

Sujet: NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV

UniProt: P0DTC9, P59595