MHC II (I-A/I-E) anticorps (violetFluor™ 450)

N° du produit ABIN7278570

Détail du produit anti-MHC II (I-A/I-E) anticorps (violetFluor™ 450)

Antigène: MHC II (I-A/I-E)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC II (I-A/I-E) est conjugé à/à la violetFluor™ 450

Application: Flow Cytometry (FACS)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Clone: M5-114-15-2

Isotype: IgG2b kappa

Information d'application

Indications d'application: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.

Commentaires:

0.2 mg/mL

Restrictions: For Research Use only

Stockage

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: 2-8°C protected from light

Date de péremption: 12 months

Détails sur MHC II (I-A/I-E)

Antigène: MHC II (I-A/I-E)

Autre désignation: MHC Class II (I-A/I-E) (MHC II (I-A/I-E) Produits)

Sujet: The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

ID gène: 14961, 14969

UniProt: P14483