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Ly6g anticorps (violetFluor™ 450)

Ly6g Reactivité: Souris FACS Hôte: Rat Monoclonal RB6-8C5 violetFluor™ 450
N° du produit ABIN7278573
  • Antigène Tous les produits Ly6g
    Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
    Reactivité
    • 90
    • 13
    • 1
    Souris
    Hôte
    • 70
    • 19
    • 1
    Rat
    Clonalité
    • 70
    • 18
    • 1
    Monoclonal
    Conjugué
    • 24
    • 8
    • 8
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Ly6g est conjugé à/à la violetFluor™ 450
    Application
    • 67
    • 33
    • 23
    • 22
    • 21
    • 15
    • 11
    • 10
    • 9
    • 8
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Purification
    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
    Clone
    RB6-8C5
    Isotype
    IgG2b kappa
  • Indications d'application
    This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
    Commentaires

    0.2 mg/mL

    Restrictions
    For Research Use only
  • Buffer
    10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    2-8°C protected from light
    Date de péremption
    12 months
  • Antigène
    Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
    Autre désignation
    Ly-6G (Gr-1) (Ly6g Produits)
    Synonymes
    anticorps Gr-1, anticorps Gr1, anticorps Ly-6G, anticorps lymphocyte antigen 6 complex, locus G, anticorps Ly6g
    Sujet
    The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
    ID gène
    17067, 100041546, 546644
    UniProt
    P0CW02, P0CW03
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