Ly6g anticorps (violetFluor™ 500)

N° du produit ABIN7278615

Détail du produit anti-Ly6g anticorps (violetFluor™ 500)

Antigène: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Ly6g est conjugé à/à la violetFluor™ 500

Application: Flow Cytometry (FACS)

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Clone: 1A8

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500 or Pacific Orange®.

Commentaires:

0.2 mg/mL

Restrictions: For Research Use only

Stockage

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: 2-8°C protected from light

Date de péremption: 12 months

Détails sur Ly6g

Antigène: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Autre désignation: Ly-6G (Ly6g Produits)

Synonymes: anticorps Gr-1, anticorps Gr1, anticorps Ly-6G, anticorps lymphocyte antigen 6 complex, locus G, anticorps Ly6g

Sujet: The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399 -2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

ID gène: 546644