Histone H2A anticorps (ubLys119)

N° du produit ABIN6971779

Détail du produit anti-Histone H2A anticorps

Antigène: Histone H2A

Épitope: ubLys119

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H2A est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Attributs du produit: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies. Histone H2AK119ub antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Affinity Purified

Immunogène: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Histone H2A

Antigène: Histone H2A

Abstract: Histone H2A Produits

Synonymes: anticorps 5499, anticorps CG5499, anticorps Dmel\\CG5499, anticorps H2A, anticorps H2A.F/Z, anticorps H2A.X, anticorps H2A.Z, anticorps H2AV, anticorps H2AV_DROM, anticorps H2AX, anticorps H2AZ, anticorps H2Av, anticorps H2AvD, anticorps H2a.V, anticorps H2av, anticorps H2avD, anticorps HIS, anticorps HIS2AV, anticorps HIS2AVD, anticorps His, anticorps His2, anticorps His2AV, anticorps His2AvD, anticorps HisH2Av, anticorps Hist, anticorps Hist2av, anticorps gamma-H2Av, anticorps gamma-HIS2AV, anticorps gamma-His2Av, anticorps gammaH2Av, anticorps h2AvD, anticorps his, anticorps l(3)05146, anticorps l(3)810, anticorps l(3)97Dd, anticorps l(3)L1602, anticorps DDBDRAFT_0205974, anticorps DDBDRAFT_0216271, anticorps DDB_0205974, anticorps DDB_0216271, anticorps RGD1564767, anticorps H2a-221, anticorps H2A-III, anticorps HIST1H2AA, anticorps Histone H2A variant, anticorps putative histone H2A, anticorps histone H2A, anticorps histone cluster 1 H2A family member F, anticorps histone H2A-like, anticorps histone cluster 1, H2af, anticorps histone2A1, anticorps histone cluster 1, H2A, III, anticorps histone cluster 1, H2ac, anticorps His2Av, anticorps LMJF_17_0280, anticorps H2AX, anticorps Hist1h2af, anticorps LOC100425378, anticorps his2a1, anticorps HIST1H2A3, anticorps HIST1H2AC, anticorps h2a-A

Poids moléculaire: 23 kDa

NCBI Accession: NP_003508