Histone 3 anticorps (3meLys27)
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- Antigène Voir toutes Histone 3 (H3) Anticorps
- Histone 3 (H3)
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Épitope
- 3meLys27
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Histone 3 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)
- Attributs du produit
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression. Histone H3K27me3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, CUT&Tag, Dot blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
- Purification
- Protein A Chromatography
- Immunogène
- This antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.
- Isotype
- IgG
- Top Product
- Discover our top product H3 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." dans: Nature, (2022) (PubMed).
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SARS-CoV-2 disrupts host epigenetic regulation via histone mimicry." dans: Nature, (2022) (PubMed).
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- Antigène
- Histone 3 (H3)
- Autre désignation
- Histone H3 (H3 Produits)
- Synonymes
- anticorps H-3, anticorps histocompatibility 3, anticorps H3
- Poids moléculaire
- 17 kDa
- NCBI Accession
- NP_003522
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