OGT anticorps (N-Term)

N° du produit ABIN6972463

Détail du produit anti-OGT anticorps

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Épitope: N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OGT est non-conjugé

Application: Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Attributs du produit: OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression. OGT/O-GlcNAc transferase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogène: This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur OGT

Antigène: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Autre désignation: OGT/O-GlcNAc transferase (OGT Produits)

Synonymes: anticorps BcDNA:GH04245, anticorps CG10392, anticorps Dmel\\CG10392, anticorps OGT, anticorps Ogt, anticorps P1201, anticorps SXC, anticorps Sxc, anticorps l(2)02637, anticorps l(2)NC130, anticorps sxc/Ogt, anticorps fm81g08, anticorps ogt, anticorps wu:fc12b01, anticorps wu:fm81g08, anticorps HRNT1, anticorps O-GLCNAC, anticorps 1110038P24Rik, anticorps 4831420N21Rik, anticorps AI115525, anticorps Ogtl, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog, anticorps super sex combs, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase, anticorps O-linked GlcNAc transferase, anticorps o-linked GlcNAc transferase, anticorps O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), anticorps ogt.L, anticorps sxc, anticorps ogt.1, anticorps OGT, anticorps ogt, anticorps GL50803_12081, anticorps GL50803_41701, anticorps THAPSDRAFT_264441, anticorps spyA, anticorps Ogt

Poids moléculaire: 120 kDa

NCBI Accession: NP_858058

Pathways: Regulation of Carbohydrate Metabolic Process