XAF1 anticorps (AA 230-280)

N° du produit ABIN6990495

Détail du produit anti-XAF1 anticorps

Antigène: XAF1 (XiAP Associated Factor 1 (XAF1))

Épitope: AA 230-280

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XAF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purification: XAF-1 Antibody is affinity chromatography purified via peptide column.

Immunogène: XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1. The immunogen is located within amino acids 230 - 280 of XAF-1.

Isotype: IgG

Information d'application

Indications d'application: XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 0.5 - 1 μ,g/mL. Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN). For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: XAF-1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: XAF-1antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur XAF1

Antigène: XAF1 (XiAP Associated Factor 1 (XAF1))

Autre désignation: XAF-1 (XAF1 Produits)

Sujet: XAF-1 Antibody: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Poids moléculaire: Predicted: 31, 33 kDa

Observed: 31 kDa

ID gène: 54739

UniProt: Q6GPH4