Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Specificité
KIF5 has at least three known isoforms, this antibody recognizes all three.
Purification
KIF5 Antibody is affinity chromatography purified via peptide column.
Immunogène
KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the center of human KIF5. The immunogen is located within amino acids 330 - 380 of KIF5.
KIF5A
Reactivité: Humain
WB, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
KIF5 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
KIF5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
KIF5 Antibody: The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the three isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.