Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificité
At least three isoforms of KLRA1 are known to exist, this antibody recognizes only the long isoform.
Purification
KLRA1 Antibody is affinity chromatography purified via peptide column.
Immunogène
KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the center of human KLRA1. The immunogen is located within amino acids 140 - 190 of KLRA1.
KLRA1
Reactivité: Humain
WB, ELISA, IF, IC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 4 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
KLRA1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
KLRA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
KLRA1
(Killer Cell Lectin-Like Receptor, Subfamily A, Member 1 (KLRA1))
KLRA1 Antibody: KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells.