Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
At least two isoforms of LRFN5 are known to exist. This antibody is predicted to not cross-react with other members of the LRFN family.
Purification
LRFN5 Antibody is affinity chromatography purified via peptide column.
Immunogène
LRFN5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LRFN5. The immunogen is located within amino acids 650 - 700 of LRFN5.
LRFN5
Reactivité: Mammifères
IHC, ISt
Hôte: Souris
Monoclonal
N140A-12
unconjugated
Indications d'application
LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
LRFN5 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
LRFN5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
LRFN5
(Leucine Rich Repeat and Fibronectin Type III Domain Containing 5 (LRFN5))
anticorps C14orf146, anticorps FIGLER8, anticorps SALM5, anticorps AI427653, anticorps AI604817, anticorps C130061B21, anticorps mKIAA4208, anticorps leucine rich repeat and fibronectin type III domain containing 5, anticorps LRFN5, anticorps Lrfn5
Sujet
LRFN5 Antibody: LRFN5 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system.