Western Blotting (WB), ELISA, Immunofluorescence (IF)
Purification
SYNPO2L Antibody is affinity chromatography purified via peptide column.
Immunogène
SYNPO2L antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SYNPO2L. The immunogen is located within amino acids 140 - 190 of SYNPO2L.
SYNPO2L antibody can be used for detection of SYNPO2L by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SYNPO2L Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
SYNPO2L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
SYNPO2L Antibody: SYNPO2L was initially identified as a novel heart-enriched gene that encodes a cytoskeletal protein highly expressed in the Z-disc of heart and skeletal muscle, associates with actin and interacts with a-actinin. It is a member of the synaptopodin family, sharing greatest homology with Synaptopodin 2. Recent studies have shown that SYNPO2L, while primarily localized to the sarcomere, can also translocate to the nucleus. A knockdown of SYNPO2L in zebrafish resulted in aberrant cardiac and skeletal muscle development and function, suggesting that it is a critical component of the sarcomere and plays an important role in muscle development.