SPRYD5 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD5 are known to exist, this antibody will detect both isoforms
Purification
SPRYD5 Antibody is affinity chromatography purified via peptide column.
Immunogène
SPRYD5 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD5. The immunogen is located within amino acids 350 - 400 of SPRYD5.
TRIM51
Reactivité: Humain
ELISA, WB
Hôte: Lapin
Polyclonal
APC
Indications d'application
SPRYD5 antibody can be used for detection of SPRYD5 by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SPRYD5 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
SPRYD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
SPRYD5 Antibody: The SPRY domain-containing protein 5 (SPRYD5) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD5 belongs to the TRIM/RBCC family and contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger. Little is known of the function of the SPRYD5 protein.