Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
Multiple isoforms of SYTL5 are known to exist. SYTL5 antibody is predicted to not cross-react with other SYTL protein family members.
Purification
SYTL5 Antibody is affinity chromatography purified via peptide column.
Immunogène
SYTL5 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human SYTL5. The immunogen is located within amino acids 30 - 80 of SYTL5.
SYTL5
Reactivité: Rat
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Cy3
Indications d'application
SYTL5 antibody can be used for detection of SYTL5 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SYTL5 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
SYTL5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
SYTL5 Antibody: SYTL5 belongs to the synaptotagmin-like (Slp) protein family, which contains a unique Slp homolog domain (SHD) at the N-terminus. The SHD functions as a binding site for Rab27A, which plays a role in protein transport. Expression of SYTL5 is restricted to placenta and liver, suggesting that it might be involved in Rab27A-dependent membrane trafficking in specific tissues. Several members of the synaptotagmin family are calcium-sensing vesicle-fusion proteins as mediators of cell migration. SYTL7 & SYTL5 were positive regulators of chemotaxis, whereas SYTL2 was a negative regulator of chemotaxis.