Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificité
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
Purification
PRICKLE1 Antibody is affinity chromatography purified via peptide column.
Immunogène
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1. The immunogen is located within the last 50 amino acids of PRICKLE1.
PRICKLE1
Reactivité: Rat
IF (cc), IF (p)
Hôte: Lapin
Polyclonal
AbBy Fluor® 680
Indications d'application
PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PRICKLE1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
PRICKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.