Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificité
KPNA5 antibody is predicted to not cross-react with other Importin alpha family members.
Purification
KPNA5 Antibody is affinity chromatography purified via peptide column.
Immunogène
KPNA5 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human KPNA5. The immunogen is located within amino acids 50 - 100 of KPNA5.
KPNA5
Reactivité: Humain
WB, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
KPNA5 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
KPNA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
KPNA5 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein imort into the nucleus.