ULK1 anticorps (AA 951-1050)

N° du produit ABIN701440

Cet anticorps Lapin Polyclonal détecte spécifiquement ULK1 dans WB, ELISA, FACS, IF (cc), IF (p), IHC (p) et IHC (fro). Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 3+ publications.

Aperçu rapide pour ULK1 anticorps (AA 951-1050) (ABIN701440)

Antigène: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ULK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-ULK1 anticorps

Épitope: AA 951-1050

Réactivité croisée: Humain, Souris, Rat

Homologie: Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human ATG1/ULK1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-ULK1 (ABIN701440)

Shen, Hou, Zhu, Zhao, Ouyang, Feng et al.: "Cortical spreading depression preconditioning mediates neuroprotection against ischemic stroke by inducing AMP-activated protein kinase-dependent autophagy in a rat cerebral ischemic/reperfusion ..." dans: Journal of neurochemistry, Vol. 140, Issue 5, pp. 799-813, (2016) (PubMed).

Gao, Wang, Yi, Kou, Xie, Song: "Activation of lysosomal degradative pathway in spinal cord tissues of carbon disulfide-treated rats." dans: Chemico-biological interactions, Vol. 219C, pp. 76-82, (2014) (PubMed).

Song, Kou, Zou, Gao, Zeng, Xie: "Involvement of autophagy in tri-ortho-cresyl phosphate- induced delayed neuropathy in hens." dans: Neurochemistry international, Vol. 64, pp. 1-8, (2013) (PubMed).

Détails sur ULK1

Antigène: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Autre désignation: ATG1/ULK1

Sujet:

Synonyms: ATG1, ATG1A, UNC51, hATG1, Unc51.1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ULK1, KIAA722

Background: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA652 and RPTOR, however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

ID gène: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy