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Recombinant LTA anticorps

LTA Reactivité: Humain ELISA, FACS, Neut, SPR Hôte: Lapin Chimeric 3F12-2D3 unconjugated Recombinant Antibody
N° du produit ABIN7072399
  • Antigène Voir toutes LTA Anticorps
    LTA (Lymphotoxin-alpha (LTA))
    Type d'anticorp
    Recombinant Antibody
    Reactivité
    • 66
    • 25
    • 9
    • 5
    • 4
    • 3
    • 3
    • 2
    Humain
    Hôte
    • 66
    • 32
    • 2
    • 1
    • 1
    Lapin
    Clonalité
    • 65
    • 37
    Chimeric
    Conjugué
    • 59
    • 12
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp LTA est non-conjugé
    Application
    • 65
    • 38
    • 29
    • 11
    • 11
    • 10
    • 8
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Neutralization (Neut), Surface Plasmon Resonance (SPR)
    Fonction
    Anti-Lymphotoxin alpha [3F12.2D3], Rabbit IgG, kappa
    Specificité
     3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE, ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTα belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTα3), or is expressed on the cell surface in complex with LTβ. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTα3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
    Attributs du produit

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2b

    Purification
    Protein A affinity purified
    Immunogène
    3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
    Clone
    3F12-2D3
    Isotype
    IgG kappa
    Top Product
    Discover our top product LTA Anticorps primaire
  • Indications d'application
    The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding.
    Commentaires

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Antigène
    LTA (Lymphotoxin-alpha (LTA))
    Autre désignation
    Lymphotoxin alpha (LTA Produits)
    Classe de substances
    Chemical
    Sujet
    LTα3 chimeric 3F12,LT, LTα, Tumor necrosis factor beta, Tumor necrosis factor β, Tumor necrosis factor-beta, tumor necrosis factor-β, TNF beta, TNF-beta, TNF β, TNF-β, Lymphotoxin-alpha, Lymphotoxin alpha
    UniProt
    P01374
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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