Recombinant LTA anticorps

N° du produit ABIN7072399

Détail du produit anti-LTA anticorps

Antigène: LTA (Lymphotoxin-alpha (LTA))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp LTA est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Neutralization (Neut), Surface Plasmon Resonance (SPR)

Fonction: Anti-Lymphotoxin alpha [3F12.2D3], Rabbit IgG, kappa

Specificité:  3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE, ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTα belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTα3), or is expressed on the cell surface in complex with LTβ. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTα3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.

Clone: 3F12-2D3

Isotype: IgG kappa

Information d'application

Indications d'application: The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur LTA

Antigène: LTA (Lymphotoxin-alpha (LTA))

Autre désignation: Lymphotoxin alpha (LTA Produits)

Classe de substances: Chemical

Sujet: LTα3 chimeric 3F12,LT, LTα, Tumor necrosis factor beta, Tumor necrosis factor β, Tumor necrosis factor-beta, tumor necrosis factor-β, TNF beta, TNF-beta, TNF β, TNF-β, Lymphotoxin-alpha, Lymphotoxin alpha

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process