Recombinant MHC, Class I anticorps

N° du produit ABIN7072426

Détail du produit anti-MHC, Class I anticorps

Antigène: MHC, Class I

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp MHC, Class I est non-conjugé

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunoassay (IA)

Fonction: Anti-MHC I [R1-21.2], Rabbit IgG, kappa

Specificité: This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.

Clone: R1-21-2

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur MHC, Class I

Antigène: MHC, Class I

Autre désignation: MHC I (MHC, Class I Produits)

Sujet: R1.21.2, H-2, MHC-I, MHC Class I