Recombinant LAG3 anticorps

N° du produit ABIN7072564

Détail du produit anti-LAG3 anticorps

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp LAG3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Fonction: Anti-LAG-3 [C9B7W], Rabbit IgG, kappa

Specificité: This antibody is specific for an epitope in the D2 domain of murine LAG-3.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.

Clone: C9B7W

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild-type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen-induced IL-2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur LAG3

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Autre désignation: Lag3 (LAG3 Produits)

Sujet: CD223, LAG3, Lymphocyte activation gene 3 protein

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints