Pol II anticorps
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- Antigène
- Pol II
- Reactivité
- Humain
- Hôte
- Souris
- Clonalité
- Monoclonal
- Application
- Immunofluorescence (IF), Western Blotting (WB)
- Réactivité croisée
- Humain
- Purification
- Purified by Protein G.
- Immunogène
- Monoclonal antibody raised in mouse against the YSPTSPS repeat in the B1 subunit of RNA polymerase II
- Isotype
- IgG1
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- Indications d'application
-
WB 1:300-5000
IF() - Restrictions
- For Research Use only
-
- Concentration
- 1 μg/μL
- Buffer
- PBS with 0.05 % sodium azide.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20°C for 12 months.
- Date de péremption
- 12 months
-
- Antigène
- Pol II
- Synonymes
- anticorps 152117_at, anticorps BG:DS00941.10, anticorps CG7885, anticorps D-rpII33, anticorps Dmel\\CG7885, anticorps Pol II, anticorps Pol II RPII33, anticorps PolII, anticorps PolIIo, anticorps RNA Pol II, anticorps RNA pol II, anticorps RNA polII, anticorps RNAP, anticorps RPB3, anticorps Rbp3, anticorps Rpb3, anticorps Rpll33, anticorps br17, anticorps l(2)34Dg, anticorps l(2)br17, anticorps l(2)k05605, anticorps l34Dg, anticorps pol II, anticorps polII, anticorps rpII33, anticorps CG3180, anticorps DmRP140, anticorps Dmel\\CG3180, anticorps H5, anticorps II, anticorps Pol IIc, anticorps PolIIc, anticorps RNA Pol II 140, anticorps RNA Pol IIc, anticorps RNAP II, anticorps RNApolII, anticorps RP140, anticorps RPB2_DROME, anticorps RPII140, anticorps RpII140[wimp], anticorps Rpb2, anticorps Rpll140, anticorps l(3)RplII140, anticorps rpII140/wimp, anticorps wimp, anticorps CG3284, anticorps Dmel\\CG3284, anticorps Pol-II, anticorps RPII15, anticorps RpII215, anticorps dRPB9, anticorps l(3)88Be, anticorps l(3)Z23, anticorps BcDNA:RH21608, anticorps CG1163, anticorps Dm6, anticorps Dmel\\CG1163, anticorps RPB6_DROME, anticorps RPII18, anticorps RpABC14, anticorps Rpll18, anticorps DNA-directed RNA polymerase II subunit RPB1, anticorps RNA polymerase II large subunit Rpb1, anticorps RNA polymerase II 33kD subunit, anticorps RNA polymerase II 140kD subunit, anticorps RNA polymerase II 15kD subunit, anticorps RNA polymerase II 18kD subunit, anticorps EDI_169920, anticorps CMU_000800, anticorps SJAG_01713, anticorps MGYG_01527, anticorps RpII33, anticorps RpII140, anticorps RpII15, anticorps RpII18
- Sujet
-
Synonyms: POLR2A, RPB1, POLR2, RPOL2
Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression
- ID gène
- 5430
- UniProt
- P24928
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