DNA2 anticorps
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- Antigène Voir toutes DNA2 Anticorps
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DNA2 est non-conjugé
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
- Purification
- Immunogen affinity purified
- Pureté
- ≥95 % as determined by SDS-PAGE
- Immunogène
- DNA replication helicase 2 homolog
- Isotype
- IgG
- Top Product
- Discover our top product DNA2 Anticorps primaire
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- Indications d'application
- WB: 1:500-1:1000, IHC: 1:50-1:500, IF: 1:10-1:100
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
- Date de péremption
- 12 months
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- Antigène
- DNA2 (DNA Replication Helicase 2 Homolog (DNA2))
- Autre désignation
- DNA2 (DNA2 Produits)
- Synonymes
- anticorps DNA2L, anticorps Dna2l, anticorps E130315B21Rik, anticorps PEOA6, anticorps hDNA2, anticorps XDna2, anticorps dna2-A, anticorps DNA replication helicase/nuclease 2, anticorps DNA replication helicase/nuclease 2 S homeolog, anticorps DNA2, anticorps Dna2, anticorps dna2.S
- Sujet
- Synonyms:DNA2, DNA2L, KIAA0083 Background:Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.
- Poids moléculaire
- 127 kDa
- ID gène
- 1763
- UniProt
- P51530
- Pathways
- Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA
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